Tau Antibody

• Catalog Number: orb1294394
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Tau
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:3000 – 1:5000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:200 – 1:300 Immunohistochemistry (Frozen) 1:200 – 1:400
• Conjugation: Unconjugated
• Target: Tau
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Tau antibody, Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294394 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human astroglioma tissue labeling Tau with orb1294394 at 1/300.

Immunofluorescent analysis. Sample: primary cortical neurons; Tau antibody (orb1294394): 1-300; Anti-rabbit 488: 1-500; Fixed: 4% paraformaldehyde at RT for 20 min.

All lanes: Anti-Tau antibody, Lysates/proteins at 45 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294394 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294394 anti-Tau antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical of frozen sections. Sample: mouse cerebellum. Green: Tau antibody (orb1294394): 1-300; Anti-rabbit 488: 1-500; DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human brain tissue labeling Tau with orb1294394 at 1/200.

Immunofluorescent analysis. Sample: primary cortical neurons; Tau antibody (orb1294394): 1-300; Anti-rabbit 488: 1-500; Fixed: 4% paraformaldehyde at RT for 20 min.

Immunohistochemical analysis of paraffin embedded mouse Alzheimer’s tissue labeling Tau with orb1294394 at 1/1000 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.