Tau Antibody: Biotin

• Catalog Number: orb699473
• Category: Antibodies
• Description: Rabbit polyclonal antibody against Truncated Tau Fragment conjugated to Biotin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant Human Tau AA297-391 (dGAE) Fibril (SPR-461)
• Concentration: 1mg/mL
• Dilution range: WB (1:1000)
• Conjugation: Biotin
• MW: 10.2 kDa
• Target: Truncated Tau Fragment (AA297-391) (dGAE)
• Entrez: 4137
• UniProt ID: P10636
• NCBI: NP_005901.2
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: Tau antibody, Neurofibrillary tangle protein antib
• Note: For research use only
• Application notes: A 1:1000 dilution of SPC-806 was sufficient for detection of tau in 15 μg of mouse, rat brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Mouse, Rat Brain showing detection of Multiple bands due to different isoforms/aggregation states Tau protein using Rabbit Anti-Tau Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse brain (15 ug). Lane 2: Rat brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple bands due to different isoforms/aggregation states. Other Band (s): 50 kDa, 65 kDa, 100 kDa.

Dot blot analysis using Rabbit Anti-Tau Polyclonal Antibody. Tissue: Recombinant Tau Monomers and Fibrils. Species: Human. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking.