TASK1 Potassium Channel Antibody: APC

• Catalog Number: orb151064
• Category: Antibodies
• Description: Mouse monoclonal to TASK1 Potassium Channel (APC). K+ channels are divided into three subclasses reflecting the number of transmembrane segments (TMS), which are designated 6TMS, 4TMS and 2TMS. Members of the 4TMS class contain two distinct pore regions and include TWIK, TREK, TRAAK and TASK. TASK channels are highly sensitive to external pH in the physiological range. TASK-1 is expressed in brain and in rat heart, with high levels of expression in the right atrium. TASK-2, mainly expressed in kidney, is localized in cortical distal tubules and collecting ducts, suggesting a role in renal K+ transport. TASK-3 from rat cerebellum shares 54% identity with TASK-1, but less than 30% identity with TASK-2 and other tandem pore K+ channels..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N374/48 (Formerly sold as S374-48)
• Tested applications: ICC, IF, IHC
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2b
• Immunogen: Fusion protein amino acids 251-411 (cytoplasmic C-terminus) of rat Acid-sensitive potassium channel protein TASK or TASK1. Mouse: 96% identity (156/161 amino acids identical). Human: 76% identity (163/161 amino acids identical). < 30% identity with TASK3.
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Conjugation: APC
• MW: 50kDa
• Target: KCNK3
• Entrez: 29553
• UniProt ID: O54912
• NCBI: NP_203694.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Alternative names: Potassium channel subfamily K member 3 antibody, K
• Note: For research use only
• Application notes: 1 µg/ml of SMC-473 was sufficient for detection of TASK1 Potassium Channel in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone N374/48. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TASK1 Potassium Channel Antibody (D) Composite.

Western Blot analysis of Rat Brain Membrane showing detection of ~50 kDa TASK1 Potassium Channel protein using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone N374/48. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain membrane. Load: 15 μg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~50 kDa.