TARBP2 Antibody

• Catalog Number: orb2636825
• Category: Antibodies
• Description: TRBP2, also known as TARBP2 (trans-activation-responsive (HIV-1) RNA binding protein 2), TRBP1 or TRBP, is a nuclear protein that contains three DRBM (double-stranded RNA-binding) domains. TRBP binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. The third DRBM motif in the C-terminus of human TRBP2 can interact with and inhibit PKR activity, thereby increasing HIV-1 long terminal repeat (LTR) expression. In addition, TRBP2 functions as a component of a Dicer-containing complex and associates with the catalytic subunit of the RNA-induced silencing complex (RISC), namely eIF2C2. TRBP2 is essential for Dicer stability and the proper assembly of RISC. This suggests that TRBP2, in association with Dicer, plays an important role in the processing of miRNAs (microRNAs).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-TARBP2-1E5
• Tested applications: FACS, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Recombinant full-length human TARBP2 protein was used as the immunogen for the TARBP2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This TARBP2 antibody is available for research use only.
• UniProt ID: Q15633
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the TARBP2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells using TARBP2 antibody (green, clone PCRP-TARBP2-1E5) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with TARBP2 antibody (blue, clone PCRP-TARBP2-1E5), and unstained cells (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using TARBP2 antibody (clone PCRP-TARBP2-1E5). These results demonstrate the foremost specificity of the PCRP-TARBP2-1E5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free TARBP2 antibody (clone PCRP-TARBP2-1E5) as confirmation of integrity and purity.