TAG-72 antibody

• Catalog Number: orb669752
• Category: Antibodies
• Description: Mouse monoclonal antibody to TAG-72
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Minretumomab (CC49 )
• Tested applications: FC, IHC, RIA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This antibody was raised by immunising BALB/c mice with TAG-72 purified from LS-174T human carcinoma xenograft.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: TAG-72
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CA 72-4; Tumor-associated antigen 72; TAG72; Tumor
• Note: For research use only
• Application notes: The reactivity of this antibody with colon cancer extract and purified TAG-72 has been confirmed in solid-phase radioimmunoassays, and its reactivity with formalin-fixed carcinoma tissue sections confirmed in immunoperoxidase studies (Muraro et al, 1988). In flow cytometric analysis of freshly-isolated human carcinoma cells, this antibody reacts more strongly than the anti-TAG-72 MAb B72.3 (Muraro et al, 1988). Equally, flow cytometric analyses using this antibody have revealed that TAG-72 is expressed by certain haematopoietic cell lines (Nicolet et al, 1997). In western blot analysis, this antibody reacts with TAG-72 (Muraro et al, 1988). A humanised version of this antibody has been shown to be safe and sensitive in detecting recurrent colorectal carcinomas in radioimmunoguided surgery (Agnese et al, 2004), while radioimmunotherapy with an iodine-131-conjugated antibody has been shown to have a significant therapeutic effect on metastatic colon carcinoma in Phase I trials (Divgi et al, 1995).
• Expiration Date: 12 months from date of receipt.