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Catalog Number | orb639619 |
---|---|
Category | Antibodies |
Description | This antibody recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of TAG-72 Ab with a GCDFP-15 Ab is valuable in the diagnosis of apocrine carcinoma. |
Species/Host | Mouse |
Clonality | Recombinant |
Clone Number | rB72.3 |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | The membrane-enriched fraction of a human breast carcinoma liver metastasis was used as the immunogen for this recombinant TAG-72 antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant TAG-72 antibody is available for research use only. |
Entrez | 182875 |
Storage | Store the recombinant TAG-72 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of human breast carcinoma with recombinant TAG-72 antibody (clone rB72.3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant TAG-72 antibody (clone rB72.3) as confirmation of integrity and purity.
FC, IF, IHC | |
Bovine, Canine, Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Bovine, Canine, Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IHC | |
Human | |
Recombinant | |
Unconjugated |
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