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Catalog Number | orb750311 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against TACSTD2 conjugated to Biotin |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | TACSTD2/2151 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG2b, kappa |
Immunogen | A portion of amino acids 31-274 from the human protein was used as the immunogen for the TROP2 antibody. |
Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
Purity | Protein G affinity chromatography |
Conjugation | Biotin |
Formula | 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This TROP2 antibody is available for research use only. |
UniProt ID | P09758 |
Storage | Store the TROP2 antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term). |
Buffer/Preservatives | 0.1 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the TROP2 antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human pancreatic carcinoma with biotinylated TROP2 antibody (clone TACSTD2/2151). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human colon carcinoma with biotinylated TROP2 antibody (clone TACSTD2/2151). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using TROP2 antibody (clone TACSTD2/2151). These results demonstrate the foremost specificity of the TACSTD2/2151 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free TROP2 antibody (clone TACSTD2/2151) as confirmation of integrity and purity.