SYP Antibody(C-term)

• Catalog Number: orb1927594
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB32966
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: IF: 1:100, IF: 1:100, WB: 1:2000, IHC-P: 1:1000, IHC-P: 1:1000, FC: 1:50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 33845 Da
• Target: This SYP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-253 amino acids from the C-terminal region of human SYP.
• UniProt ID: P08247
• NCBI: NP_003170.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Synaptophysin, Major synaptic vesicle protein p38,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

SYP Antibody (C-term) western blot analysis in mouse brain and cerebellum tissue lysates (35 ug/lane).This demonstrates the SYP antibody detected the SYP protein (arrow).

Confocal immunofluorescent analysis of SYP Antibody (C-term) with mouse brain tissue followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Confocal immunofluorescent analysis of SYP Antibody (C-term) with human brain tissue followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

SYP Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

SYP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SYP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

SYP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse hippocampus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SYP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.