SYK Antibody

• Catalog Number: orb1258274
• Category: Antibodies
• Description: SYK Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein of human SYK
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 72KDA
• Target: SYK
• UniProt ID: P43405
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: SYK, spleen tyrosine kinase, HGNC:11491, p72-Syk
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SYK antibody (orb1258274) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse brain using SYK Antibody (orb1258274) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using SYK Antibody (orb1258274) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using SYK Antibody (orb1258274) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using SYK antibody (orb1258274) at dilution of 1:100. Blue: DAPI for nuclear staining.