Swine IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated

• Catalog Number: orb347984
• Category: Antibodies
• Description: Swine IgG F(ab')2 antibody (Alkaline Phosphatase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Porcine
• Isotype: IgG
• Immunogen: Swine IgG F(ab')2 fragment
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:1,000 - 1:3,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Swine IgG, Swine IgG F(ab')2 and Swine Serum. No reaction was observed against Swine IgG F(c).
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit Anti-Swine IgG F(ab')2 alkaline phosphatase
• Note: For research use only
• Application notes: Anti-Swine IgG F(ab’)2 alkaline phosphatase conjugated antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Rabbit Anti-Swine IgG F(ab')2 Antibody Alkaline Phosphatase Conjugate tested against purified Swine IgG F(ab')2 Alk Phos. Each well was coated in duplicate with 1.0 µg of Swine IgG (p/n orb2652739). The working dilution is 1:37500. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Alkaline Phosphatase Substrate Buffer and NPP Working Buffer.