survivin/BIRC5 Antibody

• Catalog Number: orb99175
• Category: Antibodies
• Description: survivin/BIRC5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Survivin recombinant protein (Position: M1-D142).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: ELISA , 0.1-0.5μg/ml, Human, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 16389 MW
• UniProt ID: O15392
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Baculoviral IAP repeat-containing protein 5;Apopto
• Note: For research use only
• Application notes: WB: The detection limit for BIRC5 is approximately 0.5ng/lane under reducing conditions. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: Recombinant Human Survivin Protein 10ng, Lane 2: Recombinant Human Survivin Protein 5ng, Lane 3: Recombinant Human Survivin Protein 2.5ng using BIRC5 antibody