Survivin/BIRC5 Antibody

• Catalog Number: orb234279
• Category: Antibodies
• Description: Survivin/BIRC5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived mouse Survivin recombinant protein (Position: M1-A140). Mouse Survivin shares 85% and 91% amino acid (aa) sequence identity with human and rat Survivin, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 16298 MW
• UniProt ID: O70201
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Baculoviral IAP repeat-containing protein 5;Apopto
• Note: For research use only
• Application notes: WB: The detection limit for Survivin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of Neuro2a cells using anti-Survivin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of Survivin using anti-Survivin antibody.Lane 1:mouse thymus tissue; 2:mouse SP2/0 cell; 3:mouse RAW264.7 cell; 4:mouse ANA-1 cell.

IHC analysis of Survivin using anti-Survivin antibody. Survivin was detected in a paraffin-embedded section of mouse testis tissue.

IHC analysis of Survivin using anti-Survivin antibody. Survivin was detected in a paraffin-embedded section of mouse lymph node tissue.