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Catalog Number | orb1294349 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Survivin |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
Dilution range | Western Blot 1:2000-1:3000 Immunofluorescence 1:400-1:800 Immunohistochemistry (Paraffin) 1:100-1:300 |
Conjugation | Unconjugated |
Target | Survivin / BIRC5 |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Survivin/BIRC5 antibody at 1/2000 dilution. Lysates at 40 μg per lane. This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294349 overnight at 4°C.
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Anti-Survivin/BIRC5 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling Survivin/BIRC5 with orb1294349 at 1/150.
Immunohistochemical analysis of paraffin embedded Human Tonsil cancer tissue labeling Survivin/BIRC5 with orb1294349 at 1/150.
ELISA, ICC, IF, WB | |
Bovine, Mouse, Porcine, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, IHC-P, WB | |
Bovine, Porcine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IHC-P, WB | |
Bovine, Canine, Equine, Guinea pig, Porcine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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