SULT1E1 Antibody / Estrogen Sulfotransferase

• Catalog Number: orb2642733
• Category: Antibodies
• Description: The soluble sulfotransferases contribute to the elimination of xenobiotics, the activation of procarcinogens and the regulation of hormones by catalyzing the sulfate conjugation of these substances. SULT1E1, also known as STE, is a 294 amino acid member of the SULT1 family. Localized to the cytoplasm and expressed in intestine, liver and kidney, SULT1E1 exists as a homodimer that is thought to control estrogen receptor (ER) levels by sulfurylating free estradiol. Defects in the gene encoding SULT1E1 are associated with an increased risk for endometrial cancer, suggesting a role for SULT1E1 in tumorigenesis.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CPTC-SULT1E1-1
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the SULT1E1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This SULT1E1 antibody is available for research use only.
• UniProt ID: P49888
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the SULT1E1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human liver lysate with SULT1E1 antibody (clone CPTC-SULT1E1-1). Expected molecular weight ~33-35 kDa.

IHC staining of FFPE human small intestine with SULT1E1 antibody. Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human lymph node with SULT1E1 antibody. Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.