STXBP1 Antibody

• Catalog Number: orb1248794
• Category: Antibodies
• Description: STXBP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-SRVSFEDQAPTME
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Human and Mouse Brain lysates (calculated MW of 68.7kDa according to NP_003156.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Brain cortex shows vesicular staining in neuronal bodies. Recommended concentration: 5-10ug/ml. This antibody has been successfully used in IHC: Meijer M et al, PLoS One. 2015 Sep 25;10(9):e0138950. PMID: 26407320.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: STXBP1
• UniProt ID: P61764
• NCBI: NP_003156.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: STXBP1, MUNC18-1, UNC18, hUNC18, syntaxin binding
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Human and Mouse Brain lysates (calculated MW of 68.7kDa according to NP_003156.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Brain cortex shows vesicular staining in neuronal bodies. Recommended concentration: 5-10ug/ml. This antibody has been successfully used in IHC: Meijer M et al, PLoS One. 2015 Sep 25;10(9):e0138950. PMID: 26407320.
• Expiration Date: 12 months from date of receipt.

orb1248794 (0.1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248794 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.