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Catalog Number | orb345502 |
---|---|
Category | Antibodies |
Description | Sts-1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of mouse Sts-1. |
Concentration | 0.95 mg/mL |
Dilution range | ELISA: 1:2,000 - 1:10,000, IHC: 4µg/ml, IF: User Optimized, WB: 1:500 - 1:3,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This affinity purified antibody is directed against mouse Sts-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, rat and dog based on 100% homology for the immunogen sequence. Cross reactivity with Sts-1 homologues from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q8TF42 |
NCBI | 24497612 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Sts-1 antibody, Sts1, Sts 1, Ubiquitin Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry with anti-Sts-1 antibody showing Sts-1 staining of histiocytic elements in cytoplasm of human tonsil at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-Sts-1 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3'3-diamino-benzidine substrate (DAB).
Western blot using Biorbyt's Affinity Purified anti-Sts-1 antibody shows detection of a band ~70 kDa corresponding to mouse Sts-1. Approximately 1.0 µg of recombinant (truncated) Sts-1-GST was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 0.3 ug/ml overnight at 4°C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Zebrafish | |
Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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