Streptavidin Peroxidase Conjugated

• Catalog Number: orb348767
• Category: Tools
• Description: Streptavidin, a bacterial protein isolated from
• Tested applications: DOT, ELISA, IF, IHC, WB
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, IF: User Optimized, WB: 1:10,000 - 1:40,000
• Form/Appearance: Lyophilized
• Purity: Streptavidin-HRP was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.
• Conjugation: HRP
• Hazard Information: Non-Toxic
• UniProt ID: P22629
• NCBI: CAA00084.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Streptavidin Peroxidase conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: HRP-SA, Horseradish Peroxidase conjugated S avidin
• Note: For research use only
• Application notes: Streptavidin Peroxidase is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Western Blotting, and ELISA experiment formats in combination with the proper substrate.
• Expiration Date: 12 months from date of receipt.

Dot Blot Results of Streptavidin Peroxidase Conjugate. Row A: BSA-Biotin Conjugated. Row B: BSA. Sample dilutions: 1- 100 ng, 2- 33.33 ng, 3- 11.11 ng, 4-3.7 ng, 5- 1.23 ng. Streptavidin Peroxidase Conjugated at 1.0 µg/mL for 30 mins at RT.

Western Blot of Peroxidase Conjugated Streptavidin. Lane 1: Human IL-7. Load: 50 ng per lane. Primary antibody: Human IL-7 Biotin Conjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase Conjugated Streptavidin at 1:40000 for 30 min at RT. Block: 5% BLOTTO 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-7. Other band(s): none.

Western Blot Results using Streptavidin Peroxidase Conjugate and Goat Anti-GST Biotin Conjugate Antibodies. Lane 1: Opal prestained molecular weight ladder. Lane 2: GST (p/n orb345956) [0.05 µg]. Primary Antibody: Goat Anti-GST Biotin Conjugate (p/n orb345320) at 1.0 µg/mL overnight at 4°C. Secondary Antibody; Streptavidin Peroxidase Conjugate at 1:40000 for 30 mins at RT.Block: Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) for 30 mins at RT. Exp: 5 sec.