Streptavidin antibody (Texas Red)

• Catalog Number: orb344780
• Category: Antibodies
• Description: Streptavidin antibody (Texas Red)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: Streptavidin (Streptomyces avidinii)
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: User Optimized, FC: User Optimized, IF: 1:100-1:500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Streptavidin Antibody Texas Red Conjugated was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.
• Conjugation: Texas Red
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Streptavidin Antibody Texas Red Conjug
• Note: For research use only
• Application notes: Streptavidin Antibody Texas Red Conjugated antibody has been tested by dot blot and western blot and are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Biorbyt Rabbit anti Streptavidin and Biotin conjugated Rabbit anti-trypsin inhibitor antibody were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit (1:10K 1.5 hr RT in orb348637).

Dot Blot results of Rabbit Anti-Streptavidin Texas Red™ Conjugated. Antigen: Streptavidin. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Streptavidin Texas Red™ 10 µg/mL for 1 hr at RT. Secondary Antibody: none.