Streptavidin antibody (Peroxidase)

• Catalog Number: orb344727
• Category: Antibodies
• Description: Streptavidin antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: Streptavidin [Streptomyces avidinii]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000-1:10,000
• Form/Appearance: Lyophilized
• Purity: Streptavidin Antibody Peroxidase Conjugate is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii]. No cross reactivity occurs against avidin.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Streptavidin Antibody HRP Conjugation,
• Note: For research use only
• Application notes: Anti-Streptavidin Peroxidase antibody has been tested by ELISA and western blot. This product has been assayed against 1.0 ug of Streptavidin in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) for 30 minutes at room temperature. Optimal concentrations in immunoassays should be determined by researcher.
• Expiration Date: 12 months from date of receipt.

Western Blot Results of Rabbit Anti-Streptavidin Antibody Peroxidase Conjugated. Lane 1: Streptavidin Reduced [0.05 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Streptavidin Non-Reduced [0.05 µg]. Primary Antibody: Anti-Streptavidin Peroxidase at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase (p/n orb347654) at 1:70000 for 30 mins at RT. Blocking Buffer: BlockOut® Universal Blocking Buffer (p/n orb348644) for 1 hour at RT. Expect: Reduced- monomer ~17 kDa; Non-Reduced- tetramer ~45 kDa. Exposure: 5sec.