Streptavidin antibody

• Catalog Number: orb750607
• Category: Antibodies
• Description: Streptavidin antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Bacteria
• Isotype: Antiserum
• Immunogen: Streptavidin (Streptomyces avidinii)
• Concentration: 70 mg/mL
• Dilution range: ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
• Form/Appearance: Lyophilized
• Purity: Anti-STREPTAVIDIN antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Streptavidin. No cross reactivity occurs against Avidin.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Streptavidin Antibody, rabbit anti Str
• Note: For research use only
• Application notes: Anti-Streptavidin Antibody has been tested by ELISA, western blot, and dot blot and is suitable for immunoprecipitation and most immunological methods requiring high titer and specificity.
• Expiration Date: 12 months from date of receipt.

Biorbyt Rabbit anti Streptavidin and Biotin conjugated Rabbit anti-trypsin inhibitor antibody were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit.

Dot Blot showing the detection of Streptavidin. A three-fold serial dilution of DyLight™ 488 Conjugated Streptavidin starting at 200 ng was spotted onto 0.45 µM nitrocellulose and blocked in 1% BSA-TTBS (p/n orb348540, diluted to 1X) 30 min at 20°C. Anti-Streptavidin (RABBIT) Antibody (p/n orb750607) was incubated in Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) at 1:1000 for 1 Hour at 20°C. An HRP Gt-a-Rb secondary antibody (p/n orb347654) was incubated at 1:40000 for 30 min at 20°C.

Western Blot of Rabbit anti-Streptavidin Antibody. Lane 1: Streptavidin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Streptavidin Antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 13.8 kDa, 13.8 kDa. Streptavidin exhibits a homotetramer structure with four essentially identical polypeptide chains. Pictured is reduced protein.