Streptavidin antibody

• Catalog Number: orb344582
• Category: Antibodies
• Description: Streptavidin antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: Streptavidin [Streptomyces avidinii]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:200,000, IHC: 1:1,500 - 1:5,000, WB: 1:3,000 - 1:12,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Streptavidin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii]. No cross reactivity occurs against Avidin.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Streptavidin Antibody, rabbit anti Str
• Note: For research use only
• Application notes: Anti-Streptavidin antibody has been tested by ELISA, SDS-Page, and dot blot and is suitable for western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Biorbyt Rabbit anti Streptavidin and Biotin conjugated Rabbit anti-trypsin inhibitor antibody were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit (1:10K 1.5 hr RT in orb348637).

Dot Blot showing the detection of Streptavidin. A three-fold serial dilution of DyLight™ 488 Conjugated Streptavidin starting at 200 ng was spotted onto 0.45 µM nitrocellulose and blocked in 1% BSA-TTBS (p/n orb348540, diluted to 1X) 30 min at 20°C. Anti-Streptavidin (RABBIT) Antibody (p/n orb344582) was incubated in Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) at 1:1000 for 1 Hour at 20°C. An HRP Gt-a-Rb secondary antibody (p/n orb347654) was incubated at 1:40000 for 30 min at 20°C.

Western Blot of Rabbit Anti-Streptavidin Antibody. Lane 1: Streptavidin - Reduced (p/n orb348765) [0.05 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Streptavidin - Non-Reduced (p/n orb348765) [0.05 µg]. Primary Antibody: Rabbit Anti-Streptavidin at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase (p/n orb347654) at 1:70000 for 30 mins at RT. Block: Blocking Buffer for Fluorescent Western Blotting (p/n orb348637) for 60 mins at RT. Expect: ~13 kDa reduced, ~60 kDa non-reduced.

Western Blot results of Rabbit Anti-Streptavidin Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: Streptavidin (p/n orb348765). Load: 50 ng. Primary Antibody: Rabbit Anti-Streptavidin at 1:1000 overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n orb347654) at 1:70000 for 30 min at RT. Blocking: BlockOut (p/n orb348644) for 30 min at RT.