Streptavidin antibody (FITC)

• Catalog Number: orb344715
• Category: Antibodies
• Description: Streptavidin antibody (FITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: Streptavidin (Streptomyces avidinii)
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: User Optimized, FC: User Optimized, IF: 1:500-1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Streptavidin Antibody Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.
• Conjugation: FITC
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Streptavidin Antibody FITC Conjugation
• Note: For research use only
• Application notes: Anti-Streptavidin Antibody Fluorescein Conjugated has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Biorbyt Rabbit anti Streptavidin and Biotin conjugated Rabbit anti-trypsin inhibitor antibody were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit (1:10K 1.5 hr RT in orb348637).