Streptavidin antibody (Biotin)

• Catalog Number: orb344756
• Category: Antibodies
• Description: Streptavidin antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: Streptavidin (Streptomyces avidinii)
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000-1:20,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.
• Conjugation: Biotin
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Streptavidin Antibody biotin Conjugati
• Note: For research use only
• Application notes: Biotin Conjugated Anti-Streptavidin Antibody has been tested by dot blot and is suitable to be assayed by ELISA for the detection of streptavidin in a standard ELISA using Peroxidase as a reporter. A working dilution of 1:10,000 to 1:400,000 of the reconstitution concentration is suggested for this product. Optimization of the concentration in immunoassays should be performed by the researcher.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Rabbit anti-Streptavidin Biotin Conjugated. Antigen: Lane 1 - Biotin conjugated Streptavidin Antibody. Lane 2 - Streptavidin Antibody. Load: 3-fold serial dilution starting at 200 ng. Secondary antibody: HRP Streptavidin at 1:40000 for 60 min at RT. Block: 1% BSA-TTBS 30 min at RT.

Western Blot of Rabbit Anti-Trypsin Inhibitor Antibody and Rabbit Anti-Streptavidin Antibody. Lane 1: Trypsin Inhibitor reduced. Lane 2: Trypsin Inhibitor non-reduced. Lane 3: Streptavidin reduced. Lane 4: Streptavidin non-reduced. Load: ~1 ug per lane. Primary antibody: Primary antibody at 1:1000 for overnight at 4°C. Secondary antibody: Dylight 649 conjugated Donkey anti rabbit secondary antibody at 1:10000 for 45 min at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: 18.8 kDa, ~15 kDa for Streptavidin, 24 kDa, ~20 kDa for Trypsin Inhibitor. Other band(s): none.