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Catalog Number | orb344755 |
---|---|
Category | Antibodies |
Description | Streptavidin antibody (Biotin) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | DOT, ELISA |
Reactivity | Bacteria |
Isotype | IgG |
Immunogen | Streptavidin (Streptomyces avidinii) |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:2,000-1:20,000 |
Form/Appearance | Lyophilized |
Purity | Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin. |
Conjugation | Biotin |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Streptavidin Antibody biotin Conjugati Read more... |
Note | For research use only |
Application notes | Biotin Conjugated Anti-Streptavidin Antibody has been tested by dot blot and is suitable to be assayed by ELISA for the detection of streptavidin in a standard ELISA using Peroxidase as a reporter. A working dilution of 1:10,000 to 1:400,000 of the reconstitution concentration is suggested for this product. Optimization of the concentration in immunoassays should be performed by the researcher. |
Expiration Date | 12 months from date of receipt. |
Dot Blot of Rabbit anti-Streptavidin Biotin Conjugated. Antigen: Lane 1 - Biotin conjugated Streptavidin Antibody. Lane 2 - Streptavidin Antibody. Load: 3-fold serial dilution starting at 200 ng. Secondary antibody: HRP Streptavidin at 1:40000 for 60 min at RT. Block: 1% BSA-TTBS 30 min at RT.
Western Blot of Rabbit Anti-Trypsin Inhibitor Antibody and Rabbit Anti-Streptavidin Antibody. Lane 1: Trypsin Inhibitor reduced. Lane 2: Trypsin Inhibitor non-reduced. Lane 3: Streptavidin reduced. Lane 4: Streptavidin non-reduced. Load: ~1 ug per lane. Primary antibody: Primary antibody at 1:1000 for overnight at 4°C. Secondary antibody: Dylight 649 conjugated Donkey anti rabbit secondary antibody at 1:10000 for 45 min at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: 18.8 kDa, ~15 kDa for Streptavidin, 24 kDa, ~20 kDa for Trypsin Inhibitor. Other band(s): none.
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