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Item 1 of 5
Item 1 of 5
STK11 (LKB1) Antibody (N-term I29)
Catalog Number: orb1929449
| Catalog Number | orb1929449 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB3547 |
| Tested applications | FC, IF, IHC-P, WB |
| Predicted Reactivity | Mouse, Other, Rat |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 48636 Da |
| Target | This STK11 (LKB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-44 amino acids from the N-terminal region of human STK11 (LKB1). |
| UniProt ID | Q15831 |
| NCBI | NP_000446.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Serine/threonine-protein kinase STK11, Liver kinas Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
STK11 (LKB1) Antibody (N-term I29) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of STK11 (LKB1) Antibody (N-term I29) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
STK11 Antibody (I29) western blot analysis in T47D cell line lysates (35 ug/lane). This demonstrates the STK11 antibody detected the STK11 protein (arrow).
Western blot analysis of STK11 (arrow) using rabbit polyclonal STK11 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the STK11 gene (Lane 2).
