STING1 antibody

• Catalog Number: orb2276227
• Category: Antibodies
• Description: STING1 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-515M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG2c, kappa
• Immunogen: Recombinant fragment (around aa190-290) of human TMEM173 protein (exact sequence is proprietary)
• Dilution range: 1:100-1:201
• Conjugation: Unconjugated
• UniProt ID: Q86WV6
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Endoplasmic reticulum interferon stimulator; ERIS;
• Note: For research use only
• Application notes: Positive Control: Adrenal gland: Endothelial cells and macrophages should show a moderate to strong STING positivity. Negative Control: Adrenal gland: Adrenocortical and medullary cells should not show STING staining (Note: in case of tissue damage aberrant STING staining may occur). Cellular Localization: Cytoplasm Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Adrenal gland – STING staining of endothelial cells and of macrophages STING immunohistochemistry.

Appendix mucosa – STING staining of inflammatory cells predominates in the interfollicular zone STING immunohistochemistry.

Pancreas – Ductal adenocarcinoma with strong STING positivity of all tumor cells STING1 immunohistochemistry.