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Catalog Number | orb308860 |
---|---|
Category | Antibodies |
Description | Stefin B/CSTB Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human Stefin B recombinant protein (Position: M1-F98). Human Stefin B shares 78.6 % amino acid (aa) sequence identity with both mouse and rat Stefin B. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human ELISA , 0.1-0.5μg/ml, Human, - |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 11140 MW |
UniProt ID | P04080 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Cystatin-B;CPI-B;Liver thiol proteinase inhibitor; Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U20S cells using anti-Stefin B antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of A431 cells using anti-Stefin B antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of Stefin B using anti-Stefin B antibody.Lane 1:human HeLa cell;2:human A431 cell.3:human A549 cell; 4:human HepG2 cell.
IF analysis of Stefin B using anti-Stefin B antibody. Stefin B was detected in an immunocytochemical section of A431 cells.
IHC analysis of Stefin B using anti-Stefin B antibody. Stefin B was detected in a paraffin-embedded section of human prostatic cancer tissue.
FC, ICC, IHC, IHC-Fr, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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