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Catalog Number | orb345705 |
---|---|
Category | Antibodies |
Description | STAT6 (phospho-Y641) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to residues surrounding Tyrosine Y641 of human STAT6 protein. |
Concentration | 1.13 mg/mL |
Dilution range | ELISA: 1:32,000, IHC: User Optimized, WB: 1:1000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This affinity purified antibody is directed against human STAT6 pY641 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with STAT6 pY641 protein from human at 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known. |
Conjugation | Unconjugated |
UniProt ID | P42226 |
NCBI | NP_001171549.1 |
Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-STAT6 pY641 antibody, phosphorylated S Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA, IHC, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 94.1kDa in size corresponding to STAT6 pY641 protein by western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry of Rabbit anti STAT6 pY641 Antibody. Tissue: Human breast carcinoma pH 9 (left) with negative control (right).
Western Blot of Rabbit anti-STAT6 pY641 antibody. Lane 1: unstimulated Hela cell lysate (p/n orb348668). Lane 2: IL-4 stimulated Hela cell lysate. Load: 10 µg per lane. Primary antibody: STAT6 pY641 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP goat anti-rabbit (orb347654) at 1:40000 for 30 min at RT. Block: 5% Blotto (p/n orb348624), for 30 min at RT. Predicted/Observed size: 94 kDa, 90 kDa. Other band(s): unspecific bands.
IHC-P | |
Bovine, Equine, Human, Porcine, Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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