STAT6 (phospho-Y641) antibody

• Catalog Number: orb345704
• Category: Antibodies
• Description: STAT6 (phospho-Y641) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: STAT6 phospho Y641 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to residues surrounding Tyrosine 641 of human STAT6 protein.
• Concentration: 1.13 mg/mL
• Dilution range: ELISA: 1:32,000, IHC: User Optimized, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human STAT6 pY641 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with STAT6 pY641 protein from human at 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: P42226
• NCBI: NP_001171549.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-STAT6 pY641 antibody, phosphorylated S
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, IHC, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 94.1kDa in size corresponding to STAT6 pY641 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry of Rabbit anti STAT6 pY641 Antibody. Tissue: Human breast carcinoma pH 9 (left) with negative control (right).

Western Blot of Rabbit anti-STAT6 pY641 antibody. Lane 1: unstimulated Hela cell lysate (p/n orb348668). Lane 2: IL-4 stimulated Hela cell lysate. Load: 10 µg per lane. Primary antibody: STAT6 pY641 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP goat anti-rabbit (orb347654) at 1:40000 for 30 min at RT. Block: 5% Blotto (p/n orb348624), for 30 min at RT. Predicted/Observed size: 94 kDa, 90 kDa. Other band(s): unspecific bands.