STAT6 Antibody

• Catalog Number: orb1825678
• Category: Antibodies
• Description: STAT6 is a transcription factor in the Jak/STAT signal transduction pathway responsible for mediating IL-4 immune signaling. STAT6 was recently suggested to be a reliable marker to distinguish solitary fibrous tumors from other soft tissue neoplasms. Gene fusions are common in solitary fibrous tumors. Recent next generation sequencing studies demonstrated the presence of a NAB2-STAT6 fusion, formed by an intrachromosomal inversion fusing two neighboring genes on chromosome 12q13, in 55-100% of solitary fibrous tumors, regardless of tumor morphology or anatomical site. By immunohistochemistry, nuclear STAT6 expression can discriminate solitary fibrous tumors from its morphological mimics in the meninges, including meningioma, glioblastoma, gliosarcoma, haemangioblastoma, schwannoma and haemangioma. A recent study by Cheah, et al. using the rabbit monoclonal STAT6 antibody (Clone YE361) observed expression in all solitary fibrous tumors (54/54) tested, regardless of histology, anatomical site or CD34 status. Morphological mimics of solitary fibrous tumors were negative, demonstrating 100% specificity.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: STAT6/7774R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 647-847) from the human protein was used as the immunogen for the STAT6 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This STAT6 antibody is available for research use only.
• UniProt ID: P42226
• Storage: Aliquot the STAT6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human cerebellum tissue with STAT6 antibody (clone STAT6/7774R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liposarcoma tissue with STAT6 antibody (clone STAT6/7774R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.