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    STAT5B antibody

    Catalog Number: orb340708

    DispatchUsually dispatched within 5-10 working days
    $ 413.00
    Catalog Numberorb340708
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to STAT5B
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Rat
    ImmunogenRecombinant full length protein of human STAT5B
    Dilution rangeWB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:200
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetSTAT5B
    Entrez20851, 25126, 6777
    UniProt IDP42232, P52632, P51692
    SourceRabbit
    StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesSignal transducer and activator of transcription 5
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    STAT5B antibody

    Western blot analysis of STAT5B expression in HepG2 (A), PC12 (B) whole cell lysates. (Predicted band size: 89 kD; Observed band size: 100 kD)

    STAT5B antibody

    Immunohistochemical analysis of STAT5B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    STAT5B antibody

    Immunofluorescent analysis of STAT5B staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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      ICC,  IHC-Fr,  IHC-P,  IP,  WB

      Human, Mouse, Rat

      Rabbit

      Monoclonal

      Unconjugated

      100 μl, 50 μl, 20 μl
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