STAT5 R31-Me1 antibody

• Catalog Number: orb345724
• Category: Antibodies
• Description: STAT5 R31-Me1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: STAT5A [Arg31-Methyl] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 31 of human STAT.
• Concentration: 0.57 mg/ml
• Dilution range: ELISA: 1:5000, WB: 1:500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-STAT5 R31/Me1 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for STAT5 R31/Me1 and does not reacts with other STATs surrounding aa31. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Cross-reactivity with STAT5 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P42229
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.05% (w/v) Sodium Azide
• Alternative names: rabbit anti-STAT5 methyl Arg31 antibody, rabbit an
• Note: For research use only
• Application notes: Anti-STAT5 [Arg31/Me1] antibody has been tested by Dot Blot and is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~90.6 kDa corresponding to STAT5 protein by Western Blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Dot blot of Rabbit Anti-STAT5 R31-Me1 antibody. Antigen: non-modified, monomethylated and asymmetric dimethylated forms of the immunizing peptide. Load: 100, 10, or 1 picomolar as indicated. Primary antibody: STAT5 R31-Me1 antibody at 1:1000 for 90 min at RT.