STAT5 R31-Me1 antibody

• Catalog Number: orb345723
• Category: Antibodies
• Description: STAT5 R31-Me1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: STAT5A [Arg31-Methyl] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 31 of human STAT.
• Concentration: 0.57 mg/ml
• Dilution range: ELISA: 1:5000, WB: 1:500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-STAT5 R31/Me1 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for STAT5 R31/Me1 and does not reacts with other STATs surrounding aa31. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat. Cross-reactivity with STAT5 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P42229
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.05% (w/v) Sodium Azide
• Alternative names: rabbit anti-STAT5 methyl Arg31 antibody, rabbit an
• Note: For research use only
• Application notes: Anti-STAT5 [Arg31/Me1] antibody has been tested by Dot Blot and is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~90.6 kDa corresponding to STAT5 protein by Western Blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Dot blot of Rabbit Anti-STAT5 R31-Me1 antibody. Antigen: non-modified, monomethylated and asymmetric dimethylated forms of the immunizing peptide. Load: 100, 10, or 1 picomolar as indicated. Primary antibody: STAT5 R31-Me1 antibody at 1:1000 for 90 min at RT.