STAT5 (phospho-Y694) antibody

• Catalog Number: orb344455
• Category: Antibodies
• Description: STAT5 (phospho-Y694) antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5F6.F1
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: Phospho STAT5 pY694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
• Concentration: 1mg/mL
• Dilution range: ELISA: 1:20,000, IHC: 20-40 µg/ml, IF: 1:50 - 1:1,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-STAT5 phospho Y694 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for mouse Stat5a protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5a from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P42230
• NCBI: 6755672
• Storage: Store Anti-STAT5 phospho Y694 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Phospho STAT5 pY694 Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-STAT5 pY694 Antibody, MGF antibody, Sig
• Note: For research use only
• Application notes: This monoclonal antibody has been tested by ELISA, immunofluorescence, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with mouse Stat5a phospho Y694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent STED Microscopy of Anti-Stat5 pY694 (MOUSE) Monoclonal Antibody in 3T3 cells. Anti-Stat5 pY694 at 1:500 detecting Stat5 in 3T3 cells. Staining: Stat5 pY694 protein [Red]. Tubulin [Green].

Immunohistochemistry using Biorbyt Anti-STAT5 pY694 monoclonal antibody shows detection of phosphorylated STAT5 pY694 in human breast tissue (40X). The antibody was used a dilution to 20 µg/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Western blot using Biorbyt's Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution, overnight at 4°C. For detection DyLight™800 conjugated Gt-a-Mouse IgG was used at a 1:20000 dilution for 30 min at room temperature.