STAT5 (phospho-Y694) antibody

• Catalog Number: orb344439
• Category: Antibodies
• Description: STAT5 (phospho-Y694) antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5F6.F1
• Tested applications: ELISA, IF, IHC, Multiplex Assay, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000, IHC: 20-40 µg/ml, IF: 1:50-1:1000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Phospho STAT5 pY694 was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for mouse Stat5a protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5a from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P42230
• NCBI: 6755672
• Storage: Store STAT5 phospho Y694 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Phospho STAT5 antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-STAT5 pY694 Antibody, MGF antibody, Sig
• Note: For research use only
• Application notes: Phospho STAT5 pY694 monoclonal antibody has been tested by ELISA, western blot, immunohistochemistry, and immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent STED Microscopy of Anti-Stat5 pY694 (MOUSE) Monoclonal Antibody in 3T3 cells. Anti-Stat5 pY694 at 1:500 detecting Stat5 in 3T3 cells. Staining: Stat5 pY694 protein [Red]. Tubulin [Green].

Immunohistochemistry of Monoclonal Anti-Stat5 pY694 Antibody. Tissue: human breast tissue (40X). Fixation: formalin fixed paraffin embedded (FFPE). Antigen retrieval: steam sections in 0.1 M sodium citrate buffer, pH 6, for 20 min. Rinse with 1XTBST. Primary antibody: Anti-Stat5pY694 at 20 µg/mL. Localization: breast epithelium with moderate nuclear staining. Staining: Stat5 pY694 as precipitated red signal, with a hematoxylin purple nuclear counterstain.

Western blot using Biorbyt's Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution, overnight at 4°C. For detection DyLight™800 conjugated Gt-a-Mouse IgG was used at a 1:20000 dilution for 30 min at room temperature.