STAT3 (phospho-Y705) antibody

• Catalog Number: orb224010
• Category: Antibodies
• Description: Rabbit polyclonal antibody to STAT3 (Phospho-Y705)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, IP, WB
• Reactivity: Human, Mouse, Porcine, Rabbit, Rat, Sheep
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y705 of human STAT3 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:200, IP: 1:10-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: STAT3
• Entrez: 25125, 6774, 20848
• UniProt ID: P40763, P42227, P52631
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti APRF antibody, anti Signal transducer and act
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Pingwara, Rafał et al. IFN-λ Modulates the Migratory Capacity of Canine Mammary Tumor Cells via Regulation of the Expression of Matrix Metalloproteinases and Their Inhibitors Cells, 10, (2021)

PubMed: 33922837

Western blot analysis of STAT3 (Phospho-Y705) expression in mouse brain (A), H1792 (B) whole cell lysates. (Predicted band size: 88 kD; Observed band size: 86 kD)

Immunohistochemical analysis of STAT3 (Phospho-Y705) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.