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Catalog Number | orb500068 |
---|---|
Category | Antibodies |
Description | STAT3 (phospho-Ser727) antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | 4G1 |
Tested applications | FC, ICC, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated Synthesised phosphopeptide derived from human STAT3 around the phosphorylation site of Ser727 (PM(p-S)PR) |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-200, ICC=1:50-100, Flow-Cyt=2ug/Test, IHC-F=1:100-200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 88 kDa |
Target | phospho-STAT3 (Ser727) |
UniProt ID | P40763 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
Alternative names | STAT3 (phospho S727); p-STAT3 (phospho S727); STAT Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometry of A431.Primary Antibody (green line): Rabbit Anti-phospho-STAT3 (Ser727) antibody; Dilution:1μg /10^6 cells.
ICC analysis of A431 cell using phospho-STAT3 (Ser727) monoclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.
IHC-P analysis of rat brain using phospho-STAT3 Ser727 Polyclonal Antibody at 1:200 dilution.
Western blot:Heart (Rat);Cerebrum (Rat);Primary:Anti-phospho-STAT3 (Ser727) at 1:1000 dilution.
Western blot:Lane 1:Hela (Human) Cell;Lane 2:A549 (Human) Cell;Primary:Anti-phospho-STAT3 (Ser727) at 1:1000 dilution.
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P | |
Bovine, Canine, Gallus, Guinea pig, Mouse, Porcine, Rabbit, Sheep | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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