STAT3 Antibody (C-term S727)

• Catalog Number: orb1933498
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB38197
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:200, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 88068 Da
• Target: This STAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide from the C-terminal region of human STAT3, aa 711-743.
• UniProt ID: P40763
• NCBI: NP_998827.1, NP_644805.1, NP_003141.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Signal transducer and activator of transcription 3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

STAT3 Antibody (pS727) western blot analysis in Daudi cell line lysates (35 ug/lane). This demonstrates the STAT3 antibody detected the STAT3 protein (arrow).

Fluorescent confocal image of SY5Y cells stained with STAT3 (C-term S727) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with STAT3 (C-term S727) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).