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Catalog Number | orb344640 |
---|---|
Category | Antibodies |
Description | STAT1 (phospho-Y701) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y701 in the mouse STAT1 protein. |
Concentration | 2.3 mg/mL |
Dilution range | ELISA: 1:25,000, WB: 1:500 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was Protein A purified from monospecific antiserum. This antibody is specific for mouse Stat1 protein phosphorylated at Y701. A BLAST analysis was used to suggest cross-reactivity with Stat1 from mouse and rat based on 100% homology with the immunizing sequence. Partial reactivity is expected against Stat1 from human sources as 90% homology is noted. Cross-reactivity with Stat1 from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q99K94 |
NCBI | 114326482 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Stat1 pY701 Antibody, DKFZp686B04100 a Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat1 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse Stat1 pY701 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. |
Expiration Date | 12 months from date of receipt. |
Western blot using Biorbyt's Protein A purified anti-Stat1 pY701 antibody shows detection of phosphorylated Stat1 (indicated by arrowhead at ~91 kDa) in K562 cells after 30 min treatment with 1Ku of IFN-α (lane 2). No reactivity is seen for non-phosphorylated Stat1 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution at 4°C, overnight.
IF, IH, IP, WB | |
Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P | |
Bovine, Canine, Porcine, Rabbit, Rat, Sheep | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, IP, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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