STAT1 Antibody

• Catalog Number: orb19228
• Category: Antibodies
• Description: STAT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human STAT1 recombinant protein (Position: S2-A230). Human STAT1 shares 91.2% amino acid (aa) sequence identity with mouse STAT1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 87335 MW
• UniProt ID: P42224
• RRID: AB_10751224
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Signal transducer and activator of transcription 1
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-STAT1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of STAT1 using anti-STAT1 antibody.Lane 1:human HeLa cell;2:human PC-3 cell;3:human Caco-2 cell;4:human Raji cell.

IF analysis of STAT1 using anti-STAT1 antibody.STAT1 was detected in immunocytochemical section of U20S cell.

IF analysis of STAT1 using anti-STAT1 antibody.STAT1 was detected in immunocytochemical section of U20S cell.

IHC analysis of STAT1 using anti-STAT1 antibody.STAT1 was detected in paraffin-embedded section of human intestinal cancer tissues.