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Catalog Number | orb330132 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to SRP54 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Predicted Reactivity | Animal, Bovine, Canine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish |
Reactivity | Canine, Equine, Goat, Guinea pig, Human, Mouse, Rat, Zebrafish |
Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human SRP54 |
Concentration | 0.5 mg/ml |
Form/Appearance | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Conjugation | Unconjugated |
MW | 56kDa |
Target | SRP54 |
UniProt ID | P61011 |
Protein Sequence | Synthetic peptide located within the following region: ENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEA |
NCBI | NP_003127 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Alternative names | SCN8 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Hum. Placenta tissue using SRP54 antibody
Western blot analysis of human Placenta tissue using SRP54 antibody
Host: Rabbit, Target: SRP54, Positive control (+): MCF7 (N10), Negative control (-): 293T (2T), Antibody concentration: 1 ug/mL.
Hum. Adult Placenta.
SRP54 was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with orb330132 with 1:200 dilution. Western blot was performed using orb330132 at 1/1000 dilution, Lane 1: Control IP in HEK293 Whole Cell Lysate, Lane 2: SRP54 IP with orb330132 in HEK293 Whole Cell Lysate, Lane 3: Input of HEK293 Whole Cell Lysate.
WB Suggested Anti-SRP54 Antibody Titration: 0.2-1 ug/mL, ELISA Titer: 1:312500, Positive Control: Human Placenta.
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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