SRC Antibody

• Catalog Number: orb1261172
• Category: Antibodies
• Description: SRC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human SRC (NP_005408.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 60kDa
• Target: SRC
• UniProt ID: P12931
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: SRC, ASV, Pp60c-src, Tyrosine kinase pp60c-src, Ty
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SRC antibody (orb1261172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human lung cancer using SRC antibody (orb1261172) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using SRC antibody (orb1261172) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using SRC antibody (orb1261172) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using SRC antibody (orb1261172) at dilution of 1:100 (40x lens).