SRC Antibody

• Catalog Number: orb1253764
• Category: Antibodies
• Description: SRC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Src (Phospho-Tyr529) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y (p) -Q-P) derived from Human Src.
• Concentration: 1 mg/mL
• Dilution range: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 60 kDa
• Target: SRC
• UniProt ID: P12931
• NCBI: NP_005408.1
• Storage: Store antibody at -20°C for up to one year.
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Alternative names: ASV, SRC1, c-SRC, p60-Src, PKB, RAC, C-SRC
• Note: For research use only
• Application notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using Src (Phospho-Tyr529).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (Phospho-Tyr529). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Src (Phospho-Tyr529).