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Catalog Number | orb334627 |
---|---|
Category | Antibodies |
Description | SPTLC1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SPTLC1 (444-473aa IRVVVTVEQTEEELERAASTIKEVAQAVLL), different from the related mouse sequence by three amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 52744 MW |
UniProt ID | O15269 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Serine palmitoyltransferase 1;2.3.1.50;Long chain Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U20S cells using anti-SPTLC1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of SPTLC1 using anti-SPTLC1 antibody.Lane 1:human placenta tissue; 2:human U-87MG cell; 3:human A431 cell; 4:human PC-3 cell;5:human U20S cell;6:human HepG2 cell;7:human U-937 cell.
IF analysis of SPTLC1 using anti-SPTLC1 antibody.SPTLC1 was detected in immunocytochemical section of U20S cell.
IHC analysis of SPTLC1 using anti-SPTLC1 antibody.SPTLC1 was detected in paraffin-embedded section of human intestinal cancer tissues.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Yeast, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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