SPTBN2 Antibody / Spectrin beta III

• Catalog Number: orb606613
• Category: Antibodies
• Description: Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin and spectrin are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin I (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin III is highly homologous to both spectrin I and spectrin II. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPTBN2/1778
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this Spectrin beta III antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Spectrin beta III antibody is available for research use only.
• Entrez: 6712
• UniProt ID: O15020
• Storage: Store the Spectrin beta III antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Spectrin beta III antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human 1) HeLa, 2) PC-3 and 3) brain lysate with Spectrin beta III antibody. Predicted molecular weight ~246 kDa.

Western blot testing of human PANC-1 cell lysate with Spectrin beta III antibody. Predicted molecular weight ~246 kDa.

IHC testing of FFPE human pancreas with Spectrin beta III antibody (clone SPTBN2/1778). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of MeOH fixed human HeLa cells with Spectrin beta III antibody (clone SPTBN2/1778, green) and Reddot nuclear stain (red).

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Spectrin beta III antibody (clone SPTBN2/1778). These results demonstrate the foremost specificity of the SPTBN2/1778 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Spectrin beta III antibody (clone SPTBN2/1778) as confirmation of integrity and purity.