SPN Antibody

• Catalog Number: orb1252702
• Category: Antibodies
• Description: SPN Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: DF-T1
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Myeloblastic KG1 cells were used as the immunogen.
• Concentration: 0.2 mg/mL
• Dilution range: Western Blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SPN
• UniProt ID: P16150
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: SPN, GPL115, Leukosialin, Leukocyte sialoglycoprot
• Note: For research use only
• Application notes: Western Blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of spleen with CD43 antibody (DF-T1).

FACS staining of human lymphocytes using DF-T1 mAb (red) and isotype control antibody.

Western blot testing of human spleen lysate with CD43 antibody (clone DF-T1). Predicted molecular weight 45-115 kDa depending on glycosylation level.