SPINK1 Antibody

• Catalog Number: orb1538587
• Category: Antibodies
• Description: SPINK1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4D4
• Tested applications: ELISA, IHC, IHC-P, IP, WB
• Reactivity: Human
• Isotype: IgG2a,k
• Immunogen: SPINK1 (AAH25790, 24 aa ~ 80 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Concentration: 1 mg/ml
• Dilution range: ELISA, IHC, IHC-P (5 µg/ml), IP, WB (1:500 - 1:1000)
• Conjugation: Unconjugated
• Target: SPINK1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4. PBS, pH 7.4
• Alternative names: SPINK1, PCTT, PSTI, TCP, TATI, Spink3
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using tissue lysate and the recombinant protein used as the immunogen. IP: Immunoprecipitation was performed using antibody linked protein A beads. 40 ug of antibody per 100 ul of beads
• Expiration Date: 12 months from date of receipt.

Anti-SPINK1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

SPINK1 monoclonal antibody clone 4D4. Western blot of SPINK1 expression in human pancreas.

Detection limit for recombinant GST tagged SPINK1 is 0.03 ng/ml as a capture antibody.