SPI1 Antibody

• Catalog Number: orb1268006
• Category: Antibodies
• Description: SPI1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This SPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human SPI1.
• Concentration: batch dependent
• Dilution range: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 31 kDa
• Target: SPI1
• UniProt ID: P17947
• NCBI: P17947
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Transcription factor PU1, 31 kDa-transforming prot
• Note: For research use only
• Application notes: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody.NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). SPI1 immunoreactivity is localized to Nucleus significantly.

Western blot analysis in WiDr cell line lysates (35 ug/lane).

SPI1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.