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Catalog Number | orb1268006 |
---|---|
Category | Antibodies |
Description | SPI1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-P, WB |
Predicted Reactivity | Mouse, Porcine, Rat |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This SPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human SPI1. |
Concentration | batch dependent |
Dilution range | For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 31 kDa |
Target | SPI1 |
UniProt ID | P17947 |
NCBI | P17947 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Transcription factor PU1, 31 kDa-transforming prot Read more... |
Note | For research use only |
Application notes | For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody.NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). SPI1 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in WiDr cell line lysates (35 ug/lane).
SPI1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
IHC-P, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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