SPARC Rabbit Polyclonal Antibody

• Catalog Number: orb1291522
• Category: Antibodies
• Description: SPARC Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant human SPARC protein (18-303/303aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 33 kDa
• Target: SPARC
• UniProt ID: P09486
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AA517111; Basement membrane protein 40; BM 40; BM4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Measured by its binding ability in a indirect ELISA. Immobilized Human SPARC protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human SPARC Antibody, the EC50 is 21.47 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Human SPARC protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human SPARC Antibody, the EC50 is 72.97 ng/ml.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (orb1291522) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (orb1291522) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebellum tissue lysates, Lane 2: Mouse Kidney tissue lysates, Lane 3: Mouse B16 cell lysates, Lane 4: Rat Cerebrum tissue lysates, Primary: Anti-SPARC (orb1291522) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kDa, Observed band size: 33 kDa.