You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1259132 |
---|---|
Category | Antibodies |
Description | SPARC Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 35kDa |
Target | SPARC |
UniProt ID | P09486 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | SPARC, Basement-membrane protein 40, BM-40, Osteon Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using SPARC antibody (orb1259132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat Intestine using SPARC antibody (orb1259132) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SPARC antibody (orb1259132) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SPARC antibody (orb1259132) at dilution of 1:100 (40x lens).
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Bovine, Gallus, Mouse, Porcine, Rabbit, Rat, Xenopus | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
Filter by Rating